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Impact of periodontitis microbiota on inflammatory factors and T cell subsets in CIA mice. (A) Representative flow cytometry dot plot for Treg cells (CD4 + Foxp3 + ) in mouse spleen. (B) Representative flow cytometry dot plot for <t>Th17</t> cells (CD4 + IL‐17A + ) in mouse spleen. (C) Comparison of Treg cell proportions in spleen (percentage of CD4 + T cells). (D) Comparison of Th17 cell proportions in spleen. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.) (E) Serum IL‐6 levels in mice. (F) Serum CRP levels in mice. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01. #: CIA_P group versus CIA_H group, p < 0.05).
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Impact of periodontitis microbiota on inflammatory factors and T cell subsets in CIA mice. (A) Representative flow cytometry dot plot for Treg cells (CD4 + Foxp3 + ) in mouse spleen. (B) Representative flow cytometry dot plot for <t>Th17</t> cells (CD4 + IL‐17A + ) in mouse spleen. (C) Comparison of Treg cell proportions in spleen (percentage of CD4 + T cells). (D) Comparison of Th17 cell proportions in spleen. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.) (E) Serum IL‐6 levels in mice. (F) Serum CRP levels in mice. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01. #: CIA_P group versus CIA_H group, p < 0.05).
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Impact of periodontitis microbiota on inflammatory factors and T cell subsets in CIA mice. (A) Representative flow cytometry dot plot for Treg cells (CD4 + Foxp3 + ) in mouse spleen. (B) Representative flow cytometry dot plot for Th17 cells (CD4 + IL‐17A + ) in mouse spleen. (C) Comparison of Treg cell proportions in spleen (percentage of CD4 + T cells). (D) Comparison of Th17 cell proportions in spleen. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.) (E) Serum IL‐6 levels in mice. (F) Serum CRP levels in mice. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01. #: CIA_P group versus CIA_H group, p < 0.05).

Journal: The FASEB Journal

Article Title: Periodontitis Salivary Microbiota Exacerbates Murine Rheumatoid Arthritis via Gut Dysbiosis and Immune Dysregulation

doi: 10.1096/fj.202502610R

Figure Lengend Snippet: Impact of periodontitis microbiota on inflammatory factors and T cell subsets in CIA mice. (A) Representative flow cytometry dot plot for Treg cells (CD4 + Foxp3 + ) in mouse spleen. (B) Representative flow cytometry dot plot for Th17 cells (CD4 + IL‐17A + ) in mouse spleen. (C) Comparison of Treg cell proportions in spleen (percentage of CD4 + T cells). (D) Comparison of Th17 cell proportions in spleen. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.) (E) Serum IL‐6 levels in mice. (F) Serum CRP levels in mice. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01. #: CIA_P group versus CIA_H group, p < 0.05).

Article Snippet: Mouse Treg Cell Staining Kit and Mouse Th17 Cell Staining Kit (Liankebio, Hangzhou, China) were used.

Techniques: Flow Cytometry, Comparison